Quantification of Mitochondrial DNA Content

Name of the STSM:
Quantification of Mitochondrial DNA Content

Applicant:
H. Reyhan Tavukcuoglu
Akdeniz University, Dept. of Food Engineering
Antalya, Turkey

Host:
Dr. Evert van Schothorst
Prof. dr. Jaap Keijer
Human and Animal Phsyiology, Wageningen University
The Netherlands

Aim of the exchange:
The aim of this short term scientific mission was to improve knowledge of molecular techniques (Real-Time Q-PCR and gel electrophoresis) and collaborate with other scientific groups.

Results:
Mouse mitochondrial DNA was amplified and normalized versus nuclear DNA content using Q-PCR. PCR product length was checked using gel electrophoresis.
  
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